Ms. Harper’s project was to further support that invertebrates are highly sensitive to pollutants. Invertebrates are animals that have no backbone or spinal column, such as insects, worms, jellyfish, starfish, and snails. This year, in addition to Nitrates, she tested to see if ammonia, phosphates, or pH affected invertebrates. pH and ammonia both seemed to yield no affect, but the phosphates and nitrates both had high Pearson’s correlation number which conveys that they have negative consequences on invertebrates. Hanna performed conductivity and total dissolved solid tests to solidify and confirm her results. She sampled the invertebrates using a bio survey. The closer the pollutants came to 5 parts per million (ppm) concentration in the water the more potent they appeared to be. In this phase II experiment, the nitrate correlation number increased from -0.7 to -0.9, which illustrates that invertebrate populations are not only affected by pollutants, but these pollutants will eventually increase to a point where they cannot survive. If there was more water in the river to dilute the chemicals this could potentially solve the issue.